15597-B1DQ™
Download Genome Learn about the ATCC Genome PortalQuantitative Genomic RNA from Escherichia colibacteriophage MS2 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral.
- Product category
-
Viruses
- Product type
-
Molecular standard
Nucleic acid
- Derived from
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Escherichia coli bacteriophage MS2 (ATCC 15597-B1)
- Organism
-
Escherichia coli bacteriophage MS2
- Genome sequenced strain
- Yes
- Applications
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Assay development
- Specification range
- ≥1 x 105 copies/μL
- Volume
- 100 μL
- Product format
- Frozen
- Storage conditions
-
-70°C or colder
See Additional Product Information
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Documentation
Certificate of Analysis Download
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Certificate of Origin Download
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Certificate of Origin Request
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Molecular Grade Water
60-2450
Related Products
Escherichia coli bacteriophage MS2
15597-B1
Detailed product information
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General
- Specific applications
- ATCC Genuine Nucleics can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral.
Characteristics
- Comments
- Genomic RNA isolated from a preparation of Escherichia coli bacteriophage MS2 (ATCC 15597-B1). The source organism is also available through the ATCC catalog.
Handling information
- Handling procedure
-
- Thaw the vial at room temperature and immediately place on ice. Avoid exposing the RNA to repeated freeze-thaw cycles as it may result in degradation.
- Gently mix the sample to ensure an even distribution of material.
- Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
- Handling notes
- RNA is easily degraded. Take extra precautions against contamination by using new gloves and clean lab coats when working with RNA. Use only RNase-free lab materials when handling this product. Vortexing can damage the RNA. Gentle pipetting is highly recommended. Aliquoting is highly recommended to avoid multiple freeze-thaws, which can damage the RNA.
Quality control specifications
- Verification method
-
Whole-genome Sequencing
History
- Depositors
- ATCC
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Frequently Asked Questions
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References
Strauss H Jr., Sinsheimer RL. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J. Mol. Biol. 7: 43-54, 1963. PubMed: 13978804
Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001
Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001
Inactivation of MS2 virus in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 02/03/EPADWCTR.
Protocal for Equipment Verification Testing for Physical Removal of Microbiological and Particulate Contaminants. Washington, DC:Environmental Protection Agency;EPA EPA 05/9205/EPADWCTR.
USEPA Manuual of Method for Virology, Chapeter 16. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (N16), 2001
Water quality--Detection and enumeration of bacteriophages--Part 1: Enumeration of F-specific RNA bacteriophages. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10705-1:1995.
9224 C: Male-specific coliphage assay using Escherichia coli Famp. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA9224 - Detection of Coliphages;.
Water quality --- Detection and enumeration of bacteriophages --- Part 1: Enumeration of F-specific RNA bacteriophages [BS EN ISO 10705-1:2001]. London, UK:British Standards Institution;British Standard BS 6068-4.11:1996.
References
Curated Citations
Strauss H Jr., Sinsheimer RL. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J. Mol. Biol. 7: 43-54, 1963. PubMed: 13978804
Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001
Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001
Inactivation of MS2 virus in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 02/03/EPADWCTR.
Protocal for Equipment Verification Testing for Physical Removal of Microbiological and Particulate Contaminants. Washington, DC:Environmental Protection Agency;EPA EPA 05/9205/EPADWCTR.
View All Curated Citations for this Product
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