Using seahorse machine to measure OCR and ECAR in cancer cells (2024)

Zhang, J. (2019). Using seahorse machine to measure OCR and ECAR in cancer cells. In Methods in Molecular Biology (pp. 353-363). (Methods in Molecular Biology; Vol. 1928). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9027-6_18

Using seahorse machine to measure OCR and ECAR in cancer cells. / Zhang, Jing; Zhang, Qing.
Methods in Molecular Biology. Humana Press Inc., 2019. p. 353-363 (Methods in Molecular Biology; Vol. 1928).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Zhang, J 2019, Using seahorse machine to measure OCR and ECAR in cancer cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1928, Humana Press Inc., pp. 353-363. https://doi.org/10.1007/978-1-4939-9027-6_18

Zhang J, Zhang Q. Using seahorse machine to measure OCR and ECAR in cancer cells. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 353-363. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-9027-6_18

Zhang, Jing ; Zhang, Qing. / Using seahorse machine to measure OCR and ECAR in cancer cells. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 353-363 (Methods in Molecular Biology).

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title = "Using seahorse machine to measure OCR and ECAR in cancer cells",

abstract = "A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.",

keywords = "ECAR, Energy metabolism, Glycolysis, OCR, Oxidative phosphorylation, Seahorse XF24 Extracellular Flux analyzer",

author = "Jing Zhang and Qing Zhang",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",

year = "2019",

doi = "10.1007/978-1-4939-9027-6_18",

language = "English (US)",

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AU - Zhang, Jing

AU - Zhang, Qing

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PY - 2019

Y1 - 2019

N2 - A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.

AB - A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.

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KW - Glycolysis

KW - OCR

KW - Oxidative phosphorylation

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